1st(1st leg) WFC Rossiyanka - Umeå IK FF 0:1 (0:0)
2nd (2nd leg) Umeå IK FF - WFC Rossiyanka 1:1 (1:1)
1st(1st leg): 04/11/2009 13:00 2nd (2nd leg): 11/11/2009 18:00 Winner: Umeå IK FF
1st(1st leg) Røa IL - Zvezda 2005 Perm 0:0 (0:0)
2nd (2nd leg) Zvezda 2005 Perm - Røa IL 1:1 (0:1)
1st(1st leg): 04/11/2009 13:00 2nd (2nd leg): 11/11/2009 14:00 Winner: Røa IL
1st(1st leg) Montpellier HSC - Bayern München 0:0 (0:0)
2nd (2nd leg) Bayern München - Montpellier HSC 0:1 (0:0, 0:0) aet
1st(1st leg): 04/11/2009 17:00 2nd (2nd leg): 11/11/2009 18:00 Winner: Montpellier HSC
1st(1st leg) AC Sparta Praha - Arsenal LFC 0:3 (0:2)
2nd (2nd leg) Arsenal LFC - AC Sparta Praha 2:0 (1:0)
1st(1st leg): 04/11/2009 17:00 2nd (2nd leg): 11/11/2009 13:30 Winner: Arsenal LFC
1st(1st leg) Turbine Potsdam - Brøndby IF 1:0 (0:0)
2nd (2nd leg) Brøndby IF - Turbine Potsdam 0:4 (0:1)
1st(1st leg): 04/11/2009 18:00 2nd (2nd leg): 11/11/2009 18:00 Winner: Turbine Potsdam
1st(1st leg) SV Neulengbach - ASD Torres Calcio 1:4 (1:4)
2nd (2nd leg) ASD Torres Calcio - SV Neulengbach 4:1 (0:0)
1st(1st leg): 04/11/2009 18:00 2nd (2nd leg): 11/11/2009 13:30 Winner: ASD Torres Calcio
1st(1st leg) FCR 2001 Duisburg - Linköpings FC 1:1 (0:1)
2nd (2nd leg) Linköpings FC - FCR 2001 Duisburg 0:2 (0:0)
1st(1st leg): 04/11/2009 18:30 2nd (2nd leg): 11/11/2009 18:00 Winner: FCR 2001 Duisburg
1st(1st leg) Fortuna Hjørring - Olympique Lyonnais 0:1 (0:1)
2nd (2nd leg) Olympique Lyonnais - Fortuna Hjørring 5:0 (3:0)
1st(1st leg): 05/11/2009 18:00 2nd (2nd leg): 12/11/2009 17:00 Winner: Olympique Lyonnais