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| 23/10/2010 | 15:00 | Deveronvale FC | - | Dalbeattie Star | 1:0 | |
| 15:00 | Forres Mechanics FC | - | East Stirlingshire FC | 0:0 | ||
| 15:00 | Preston Athletic FC | - | Annan Athletic | 0:0 | ||
| 15:00 | Clyde FC | - | Berwick Rangers | 1:2 | ||
| 15:00 | Wigtown & Bladnoch | - | Buckie Thistle | 1:7 | ||
| 15:00 | Beith Juniors FC | - | Glasgow University | 7:1 | ||
| 15:00 | Nairn County FC | - | Cove Rangers | 0:1 | ||
| 15:00 | Keith FC | - | The Spartans FC | 0:3 | ||
| 15:00 | Stranraer FC | - | St. Cuthbert Wanderers | 9:0 | ||
| 15:00 | Bo'ness United | - | Queen's Park FC | 2:1 | ||
| 15:00 | Golspie Sutherland | - | Girvan FC | 1:1 | ||
| 15:00 | Montrose FC | - | Arbroath FC | 1:1 | ||
| 15:00 | Albion Rovers | - | Sunnybank FC | 0:1 | ||
| 15:00 | Edinburgh City FC | - | Threave Rovers | 2:4 | ||
| 15:00 | Elgin City | - | Brora Rangers FC | 5:3 | ||
| 30/10/2010 | 15:00 | Whitehill Welfare | - | Wick Academy FC | 4:3 | |
| decisive matches | ||||||
|---|---|---|---|---|---|---|
| 15:00 | East Stirlingshire FC | - | Forres Mechanics FC | 4:0 | ||
| 15:00 | Annan Athletic | - | Preston Athletic FC | 5:0 | ||
| 15:00 | Arbroath FC | - | Montrose FC | 2:3 aet | ||
| 01/11/2010 | 13:30 | Girvan FC | - | Golspie Sutherland | 4:0 | |
