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| 27/01/2000 | RKC Waalwijk | - | SBV Eindhoven | 2:0 (0:0) | ||
| 28/01/2000 | Helmond Sport | - | Willem II | 1:2 (0:1) | ||
| Roda JC Kerkrade | - | AFC Ajax | 1:0 (0:0) | |||
| NEC Nijmegen | - | SBV Excelsior | 1:0 (0:0) | |||
| Dordrecht '90 | - | RBC Roosendaal | 1:0 (0:0) | |||
| 29/01/2000 | PSV Eindhoven | - | Vitesse | 0:2 (0:1) | ||
| 30/01/2000 | FC Utrecht | - | Go Ahead Eagles | 5:3 (2:0) | ||
| Feyenoord | - | AZ Alkmaar | 1:3 (1:1) |
