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| 15/01/2008 | 19:00 | RKC Waalwijk | - | HFC Haarlem | 3:5 (0:0, 1:1, 1:1) pso | |
| 19:00 | FC Dordrecht | - | AGOVV | 4:3 (1:1, 2:2, 4:3) aet | ||
| 19:00 | Roda JC Kerkrade | - | SBV Excelsior | 3:0 (1:0) | ||
| 19:00 | NEC Nijmegen | - | FC Zwolle | 0:2 (0:1) | ||
| 19:45 | SV Deurne | - | Feyenoord | 0:4 (0:2) | ||
| 16/01/2008 | 19:00 | Heracles Almelo | - | FC Den Bosch | 1:0 (0:0) | |
| 19:00 | Quick Boys | - | sc Heerenveen/FC Emmen (J) | 3:1 (1:0, 1:1, 1:1) pso | ||
| 19:45 | NAC Breda | - | AFC Ajax | 4:2 (1:0) |
