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| 19/08/2000 | 11:00 | Fortuna Magdeburg/Wolmirstedt | - | FFC Flaesheim-Hillen | 1:6 | |
| 13:00 | TSV Pfersee Augsburg | - | SC 07 Bad Neuenahr | 0:5 | ||
| 15:00 | 1. FC Nürnberg | - | Bayern München | 1:5 | ||
| 16:00 | SpVgg Rehweiler-Matzenbach | - | FSV Frankfurt | 0:4 | ||
| 20/08/2000 | 10:00 | Berliner SV | - | Turbine Potsdam | 0:16 | |
| 10:00 | USV Jena | - | Brauweiler Pulheim | 0:7 | ||
| 10:00 | Hamburger SV | - | FCR Duisburg 55 | 2:1 aet | ||
| 10:00 | Turbine Potsdam II | - | SG Essen-Schönebeck | 1:4 | ||
| 10:00 | DJK FSV Schwarzbach | - | 1. FC Saarbrücken | 0:2 | ||
| 13:00 | PSV 90 Neubrandenburg | - | WSV Wolfsburg-Wendschott | 1:13 | ||
| 13:00 | Herforder SV | - | SV Neuenbrook/Rethwisch | 5:0 | ||
| 13:00 | 1. FC Saarbrücken II | - | 1. FFC Frankfurt | 0:7 | ||
| 13:00 | TuS Niederkirchen | - | SC Freiburg | 0:2 | ||
| 13:00 | SC 07 Bad Neuenahr II | - | Viktoria Neckarhausen | 1:5 | ||
| 14:00 | Victoria Gersten | - | Heike Rheine | 2:5 | ||
| 14:00 | ATS Buntentor | - | Sportfreunde Siegen | 0:5 |
