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| 1st(1st leg) | Deportivo La Coruña | - | CE Sabadell | 3:1 | ||
| 2nd (2nd leg) | CE Sabadell | - | Deportivo La Coruña | 1:0 | ||
| 1st(1st leg): 21/01/1989 | 2nd (2nd leg): 04/02/1989 | Winner: Deportivo La Coruña | ||||
| 1st(1st leg) | Real Betis | - | CD Logroñés | 5:1 | ||
| 2nd (2nd leg) | CD Logroñés | - | Real Betis | 0:0 | ||
| 1st(1st leg): 22/01/1989 | 2nd (2nd leg): 05/02/1989 | Winner: Real Betis | ||||
| 1st(1st leg) | UE Lleida | - | Cádiz CF | 1:3 | ||
| 2nd (2nd leg) | Cádiz CF | - | UE Lleida | 2:2 | ||
| 1st(1st leg): 22/01/1989 | 2nd (2nd leg): 01/02/1989 | Winner: Cádiz CF | ||||
| 1st(1st leg) | CD Málaga | - | Espanyol Barcelona | 0:0 | ||
| 2nd (2nd leg) | Espanyol Barcelona | - | CD Málaga | 1:0 | ||
| 1st(1st leg): 22/01/1989 | 2nd (2nd leg): 05/02/1989 | Winner: Espanyol Barcelona | ||||
| 1st(1st leg) | Recreativo Huelva | - | Real Murcia | 3:1 | ||
| 2nd (2nd leg) | Real Murcia | - | Recreativo Huelva | 3:2 | ||
| 1st(1st leg): 22/01/1989 | 2nd (2nd leg): 02/02/1989 | Winner: Recreativo Huelva | ||||
| 1st(1st leg) | Real Zaragoza | - | Real Valladolid | 0:1 | ||
| 2nd (2nd leg) | Real Valladolid | - | Real Zaragoza | 1:1 | ||
| 1st(1st leg): 22/01/1989 | 2nd (2nd leg): 05/02/1989 | Winner: Real Valladolid | ||||
| 1st(1st leg) | Arosa SC | - | Real Sociedad | 0:1 | ||
| 2nd (2nd leg) | Real Sociedad | - | Arosa SC | 1:0 | ||
| 1st(1st leg): 25/01/1989 | 2nd (2nd leg): 01/02/1989 | Winner: Real Sociedad | ||||
| 1st(1st leg) | Atlético Madrid | - | UD Las Palmas | 1:0 | ||
| 2nd (2nd leg) | UD Las Palmas | - | Atlético Madrid | 0:2 | ||
| 1st(1st leg): 25/01/1989 | 2nd (2nd leg): 01/02/1989 | Winner: Atlético Madrid | ||||
| 1st(1st leg) | Real Burgos | - | RCD Mallorca | 1:1 | ||
| 2nd (2nd leg) | RCD Mallorca | - | Real Burgos | 2:0 | ||
| 1st(1st leg): 25/01/1989 | 2nd (2nd leg): 01/02/1989 | Winner: RCD Mallorca | ||||
| 1st(1st leg) | FC Cartagena | - | FC Barcelona | 0:3 | ||
| 2nd (2nd leg) | FC Barcelona | - | FC Cartagena | 4:0 | ||
| 1st(1st leg): 25/01/1989 | 2nd (2nd leg): 01/02/1989 | Winner: FC Barcelona | ||||
| 1st(1st leg) | Elche CF | - | Real Madrid | 1:2 | ||
| 2nd (2nd leg) | Real Madrid | - | Elche CF | 1:1 | ||
| 1st(1st leg): 25/01/1989 | 2nd (2nd leg): 01/02/1989 | Winner: Real Madrid | ||||
| 1st(1st leg) | Xerez CD | - | RC Celta | 2:2 | ||
| 2nd (2nd leg) | RC Celta | - | Xerez CD | 4:1 | ||
| 1st(1st leg): 25/01/1989 | 2nd (2nd leg): 01/02/1989 | Winner: RC Celta | ||||
| 1st(1st leg) | CA Osasuna | - | Sevilla FC | 1:0 | ||
| 2nd (2nd leg) | Sevilla FC | - | CA Osasuna | 1:1 | ||
| 1st(1st leg): 25/01/1989 | 2nd (2nd leg): 01/02/1989 | Winner: CA Osasuna | ||||
| 1st(1st leg) | Racing Santander | - | Valencia CF | 0:0 | ||
| 2nd (2nd leg) | Valencia CF | - | Racing Santander | 2:3 | ||
| 1st(1st leg): 25/01/1989 | 2nd (2nd leg): 01/02/1989 | Winner: Racing Santander | ||||
| 1st(1st leg) | Sestao SC | - | Athletic Club | 0:0 | ||
| 2nd (2nd leg) | Athletic Club | - | Sestao SC | 1:0 | ||
| 1st(1st leg): 25/01/1989 | 2nd (2nd leg): 01/02/1989 | Winner: Athletic Club | ||||
| 1st(1st leg) | CD Tenerife | - | Sporting Gijón | 2:1 | ||
| 2nd (2nd leg) | Sporting Gijón | - | CD Tenerife | 5:1 | ||
| 1st(1st leg): 25/01/1989 | 2nd (2nd leg): 01/02/1989 | Winner: Sporting Gijón | ||||
