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| 1st(1st leg) | FK Mladá Boleslav | - | 1. FC Slovácko | 1:0 (1:0) | ||
| 2nd (2nd leg) | 1. FC Slovácko | - | FK Mladá Boleslav | 0:2 | ||
| 1st(1st leg): 04/10/2013 14:30 | 2nd (2nd leg): 11/10/2013 16:00 | Winner: FK Mladá Boleslav | ||||
| 1st(1st leg) | Viktoria Žižkov | - | Zbrojovka Brno | 1:0 | ||
| 2nd (2nd leg) | Zbrojovka Brno | - | Viktoria Žižkov | 4:0 | ||
| 1st(1st leg): 05/10/2013 13:00 | 2nd (2nd leg): 30/10/2013 17:00 | Winner: Zbrojovka Brno | ||||
| 1st(1st leg) | 1. SC Znojmo | - | AC Sparta Praha | 2:3 (1:1) | ||
| 2nd (2nd leg) | AC Sparta Praha | - | 1. SC Znojmo | 2:1 | ||
| 1st(1st leg): 05/10/2013 14:30 | 2nd (2nd leg): 05/11/2013 17:00 | Winner: AC Sparta Praha | ||||
| 1st(1st leg) | FK Baumit Jablonec | - | FK Ústí nad Labem | 1:0 (1:0) | ||
| 2nd (2nd leg) | FK Ústí nad Labem | - | FK Baumit Jablonec | 0:2 | ||
| 1st(1st leg): 05/10/2013 15:00 | 2nd (2nd leg): 29/10/2013 13:30 | Winner: FK Baumit Jablonec | ||||
| 1st(1st leg) | FK Varnsdorf | - | Slavia Praha | 0:0 (0:0) | ||
| 2nd (2nd leg) | Slavia Praha | - | FK Varnsdorf | 1:0 | ||
| 1st(1st leg): 05/10/2013 15:00 | 2nd (2nd leg): 29/10/2013 17:00 | Winner: Slavia Praha | ||||
| 1st(1st leg) | FK Dukla Praha | - | 1. FK Příbram | 4:0 (2:0) | ||
| 2nd (2nd leg) | 1. FK Příbram | - | FK Dukla Praha | 1:2 | ||
| 1st(1st leg): 05/10/2013 15:00 | 2nd (2nd leg): 29/10/2013 16:00 | Winner: FK Dukla Praha | ||||
| 1st(1st leg) | Sigma Olomouc | - | Vysočina Jihlava | 0:4 (0:2) | ||
| 2nd (2nd leg) | Vysočina Jihlava | - | Sigma Olomouc | 0:0 | ||
| 1st(1st leg): 05/10/2013 16:00 | 2nd (2nd leg): 30/10/2013 16:00 | Winner: Vysočina Jihlava | ||||
| 1st(1st leg) | TJ Jiskra Domažlice | - | Viktoria Plzeň | 0:3 | ||
| 2nd (2nd leg) | Viktoria Plzeň | - | TJ Jiskra Domažlice | 4:0 (0:0) | ||
| 1st(1st leg): 16/11/2013 10:00 | 2nd (2nd leg): 26/03/2014 15:00 | Winner: Viktoria Plzeň | ||||
