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| 1st(1st leg) | FK Baumit Jablonec | - | Slavia Praha | 1:0 | ||
| 2nd (2nd leg) | Slavia Praha | - | FK Baumit Jablonec | 2:1 | ||
| 1st(1st leg): 21/04/2010 16:00 | 2nd (2nd leg): 28/04/2010 18:15 | Winner: FK Baumit Jablonec | ||||
| 1st(1st leg) | Viktoria Plzeň | - | Sigma Olomouc | 2:2 | ||
| 2nd (2nd leg) | Sigma Olomouc | - | Viktoria Plzeň | 1:3 | ||
| 1st(1st leg): 21/04/2010 17:30 | 2nd (2nd leg): 28/04/2010 16:30 | Winner: Viktoria Plzeň | ||||
