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| 17/04/2021 | 13:30 | MFK Chrudim | - | SK Líšeň | 0:3 (0:1) | |
| 18/04/2021 | 09:15 | Viktoria Žižkov | - | FK Ústí nad Labem | 1:0 (1:0) | |
| 14:00 | Slavoj Vyšehrad | - | 1. SK Prostějov | 0:1 (0:0) | ||
| 16:00 | FC Hradec Králové | - | FK Fotbal Třinec | 1:0 (1:0) | ||
| 16:00 | Vysočina Jihlava | - | FK Dukla Praha | 0:1 (0:1) | ||
| 16:00 | FK MAS Táborsko | - | FK Blansko | 1:0 (1:0) | ||
| 16:00 | FC Sellier & Bellot Vlašim | - | FK Varnsdorf | 0:0 (0:0) |
| » Tables | Home | Away | First round | Second round |
| # | Team | M. | W | D | L | goals | Dif. | Pt. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
FC Hradec Králové | 19 | 13 | 4 | 2 | 39:16 | 23 | 43 |
| 2 |
|
SK Líšeň | 19 | 10 | 7 | 2 | 33:18 | 15 | 37 |
| 3 |
|
1. SK Prostějov | 19 | 10 | 5 | 4 | 32:25 | 7 | 35 |
| 4 |
|
FK Ústí nad Labem | 19 | 9 | 5 | 5 | 19:21 | -2 | 32 |
| 5 |
|
MFK Chrudim | 19 | 9 | 3 | 7 | 27:19 | 8 | 30 |
| 6 |
|
FC Sellier & Bellot Vlašim | 19 | 8 | 4 | 7 | 27:22 | 5 | 28 |
| 7 |
|
FK Dukla Praha | 19 | 7 | 6 | 6 | 28:22 | 6 | 27 |
| 8 |
|
Viktoria Žižkov | 19 | 8 | 2 | 9 | 26:29 | -3 | 26 |
| 9 |
|
FK Varnsdorf | 19 | 5 | 8 | 6 | 16:22 | -6 | 23 |
| 10 |
|
Vysočina Jihlava | 19 | 6 | 4 | 9 | 27:32 | -5 | 22 |
| 11 |
|
FK Blansko | 19 | 5 | 5 | 9 | 21:23 | -2 | 20 |
| 12 |
|
FK Fotbal Třinec | 19 | 5 | 5 | 9 | 22:28 | -6 | 20 |
| 13 |
|
FK MAS Táborsko | 19 | 5 | 4 | 10 | 17:22 | -5 | 19 |
| 14 |
|
Slavoj Vyšehrad | 19 | 1 | 2 | 16 | 9:44 | -35 | 5 |
| Promotion | |
| Relegation |
