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| 1st(1st leg) | Atlético Junior | - | Atlético Nacional | 1:1 (0:1) | ||
| 2nd (2nd leg) | Atlético Nacional | - | Atlético Junior | 2:4 (0:0, 0:0) pso | ||
| 1st(1st leg): 09/06/2016 01:00 | 2nd (2nd leg): 11/06/2016 21:00 | Winner: Atlético Junior | ||||
| 1st(1st leg) | Cortuluá | - | Independiente Medellín | 1:2 (1:0) | ||
| 2nd (2nd leg) | Independiente Medellín | - | Cortuluá | 8:7 (0:1, 1:2) pso | ||
| 1st(1st leg): 10/06/2016 00:30 | 2nd (2nd leg): 12/06/2016 22:30 | Winner: Independiente Medellín | ||||
