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| 1st(1st leg) | Olympique Lyonnais | - | Real Madrid | 1:0 (0:0) | ||
| 2nd (2nd leg) | Real Madrid | - | Olympique Lyonnais | 1:1 (1:0) | ||
| 1st(1st leg): 16/02/2010 19:45 | 2nd (2nd leg): 10/03/2010 19:45 | Winner: Olympique Lyonnais | ||||
| 1st(1st leg) | AC Milan | - | Manchester United | 2:3 (1:1) | ||
| 2nd (2nd leg) | Manchester United | - | AC Milan | 4:0 (1:0) | ||
| 1st(1st leg): 16/02/2010 19:45 | 2nd (2nd leg): 10/03/2010 19:45 | Winner: Manchester United | ||||
| 1st(1st leg) | Bayern München | - | ACF Fiorentina | 2:1 (1:0) | ||
| 2nd (2nd leg) | ACF Fiorentina | - | Bayern München | 3:2 (1:0) | ||
| 1st(1st leg): 17/02/2010 19:45 | 2nd (2nd leg): 09/03/2010 19:45 | Winner: Bayern München | ||||
| 1st(1st leg) | FC Porto | - | Arsenal FC | 2:1 (1:1) | ||
| 2nd (2nd leg) | Arsenal FC | - | FC Porto | 5:0 (2:0) | ||
| 1st(1st leg): 17/02/2010 19:45 | 2nd (2nd leg): 09/03/2010 19:45 | Winner: Arsenal FC | ||||
| 1st(1st leg) | VfB Stuttgart | - | FC Barcelona | 1:1 (1:0) | ||
| 2nd (2nd leg) | FC Barcelona | - | VfB Stuttgart | 4:0 (2:0) | ||
| 1st(1st leg): 23/02/2010 19:45 | 2nd (2nd leg): 17/03/2010 19:45 | Winner: FC Barcelona | ||||
| 1st(1st leg) | Olympiakos Piraeus | - | Girondins Bordeaux | 0:1 (0:1) | ||
| 2nd (2nd leg) | Girondins Bordeaux | - | Olympiakos Piraeus | 2:1 (1:0) | ||
| 1st(1st leg): 23/02/2010 19:45 | 2nd (2nd leg): 17/03/2010 19:45 | Winner: Girondins Bordeaux | ||||
| 1st(1st leg) | CSKA Moskva | - | Sevilla FC | 1:1 (0:1) | ||
| 2nd (2nd leg) | Sevilla FC | - | CSKA Moskva | 1:2 (1:1) | ||
| 1st(1st leg): 24/02/2010 17:30 | 2nd (2nd leg): 16/03/2010 19:45 | Winner: CSKA Moskva | ||||
| 1st(1st leg) | Inter | - | Chelsea FC | 2:1 (1:0) | ||
| 2nd (2nd leg) | Chelsea FC | - | Inter | 0:1 (0:0) | ||
| 1st(1st leg): 24/02/2010 19:45 | 2nd (2nd leg): 16/03/2010 19:45 | Winner: Inter | ||||
