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| 1st(1st leg) | Remo - PA | - | Figueirense - SC | 4:1 | ||
| 2nd (2nd leg) | Figueirense - SC | - | Remo - PA | 2:1 | ||
| 1st(1st leg): 14/10/2000 | 2nd (2nd leg): 19/10/2000 | Winner: Remo - PA | ||||
| 1st(1st leg) | Caxias - SC | - | Sampaio Corrêa - MA | 2:0 | ||
| 2nd (2nd leg) | Sampaio Corrêa - MA | - | Caxias - SC | 1:1 | ||
| 1st(1st leg): 14/10/2000 | 2nd (2nd leg): 18/10/2000 | Winner: Caxias - SC | ||||
| 1st(1st leg) | Paysandu - PA | - | Botafogo - SP | 3:0 | ||
| 2nd (2nd leg) | Botafogo - SP | - | Paysandu - PA | 2:1 | ||
| 1st(1st leg): 15/10/2000 | 2nd (2nd leg): 19/10/2000 | Winner: Paysandu - PA | ||||
| 1st(1st leg) | Criciúma - SC | - | Náutico - PE | 1:2 | ||
| 2nd (2nd leg) | Náutico - PE | - | Criciúma - SC | 0:0 | ||
| 1st(1st leg): 15/10/2000 | 2nd (2nd leg): 18/10/2000 | Winner: Náutico - PE | ||||
| 1st(1st leg) | Bangu - RJ | - | São Raimundo - AM | 2:1 | ||
| 2nd (2nd leg) | São Raimundo - AM | - | Bangu - RJ | 2:2 | ||
| 1st(1st leg): 15/10/2000 | 2nd (2nd leg): 18/10/2000 | Winner: Bangu - RJ | ||||
| 1st(1st leg) | Avaí - SC | - | Fortaleza - CE | 0:2 | ||
| 2nd (2nd leg) | Fortaleza - CE | - | Avaí - SC | 1:2 | ||
| 1st(1st leg): 15/10/2000 | 2nd (2nd leg): 18/10/2000 | Winner: Fortaleza - CE | ||||
| 1st(1st leg) | Anapolina - GO | - | Paraná Clube | 0:1 | ||
| 2nd (2nd leg) | Paraná Clube | - | Anapolina - GO | 2:0 | ||
| 1st(1st leg): 15/10/2000 | 2nd (2nd leg): 18/10/2000 | Winner: Paraná Clube | ||||
| 1st(1st leg) | CR Brasil - AL | - | São Caetano - SP | 1:1 | ||
| 2nd (2nd leg) | São Caetano - SP | - | CR Brasil - AL | 4:1 | ||
| 1st(1st leg): 16/10/2000 | 2nd (2nd leg): 18/10/2000 | Winner: São Caetano - SP | ||||
