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| 03/04/2001 | 15:00 | SVG Bleiburg | - | DSV Leoben | 0:3 | |
| 15:30 | ESK/Grazer (A) | - | SV Braunau | 1:4 | ||
| 15:30 | SV Hundsheim | - | BSV Bad Bleiberg | 3:2 | ||
| 15:30 | SV Langenrohr | - | Rapid Wien | 0:4 (0:0) | ||
| 17:00 | SVG Reichenau | - | VfB Admira Wacker Mödling | 1:6 | ||
| 18:00 | ASKÖ Donau Linz | - | LASK Linz | 1:3 | ||
| 18:00 | Kapfenberger SV | - | SC Untersiebenbrunn | 4:1 pso | ||
| 18:00 | SV Stockerau | - | Schwarz-Weiß Bregenz | 0:3 | ||
| 18:00 | Sturm Graz | - | Austria Wien | 4:2 aet | ||
| 18:00 | Union St. Florian | - | Austria Lustenau | 0:1 | ||
| 18:00 | First Vienna FC | - | FC Kärnten | 1:2 | ||
| 18:00 | Wolfsberger AC | - | WSG Wattens | 2:1 | ||
| 18:30 | Kremser SC | - | SV Wörgl | 3:0 | ||
| 04/04/2001 | 15:30 | FC Zeltweg | - | Grazer AK | 1:0 (0:0, 0:0) aet | |
| 17:30 | Floridsdorfer AC | - | SV Austria Salzburg | 0:2 | ||
| 18:00 | SV Ried | - | FC Tirol Innsbruck | 0:3 (0:0) |
