| 1. Round | 12/09/2020 | FC Twente | - | Fortuna Sittard | 2:0 | ||||
| 3. Round | 25/09/2020 | FC Twente | - | FC Groningen | 3:1 | ||||
| 4. Round | 03/10/2020 | FC Twente | - | FC Emmen | 1:1 | ||||
| 5. Round | 17/10/2020 | Willem II | - | FC Twente | 0:3 | ||||
| 6. Round | 24/10/2020 | FC Utrecht | - | FC Twente | 2:1 | ||||
| 7. Round | 31/10/2020 | FC Twente | - | PEC Zwolle | 5:1 |  |
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| 8. Round | 07/11/2020 | ADO Den Haag | - | FC Twente | 2:4 | ||||
| 9. Round | 22/11/2020 | FC Twente | - | PSV Eindhoven | 1:1 | ||||
| 10. Round | 27/11/2020 | FC Twente | - | RKC Waalwijk | 0:2 | ||||
| 11. Round | 05/12/2020 | AFC Ajax | - | FC Twente | 1:2 | ||||
| 12. Round | 13/12/2020 | FC Twente | - | AZ Alkmaar | 1:3 | ||||
| 13. Round | 19/12/2020 | VVV-Venlo | - | FC Twente | 1:2 | ||||
| 14. Round | 22/12/2020 | FC Twente | - | Sparta Rotterdam | 0:2 | ||||
| 17. Round | 17/01/2021 | FC Groningen | - | FC Twente | 2:2 | ||||
| 26. Round | 13/03/2021 | AZ Alkmaar | - | FC Twente | 4:1 | ||||
| 27. Round | 19/03/2021 | sc Heerenveen | - | FC Twente | 0:0 | ||||
| 31. Round | 01/05/2021 | Fortuna Sittard | - | FC Twente | 3:0 |  |
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| 32. Round | 08/05/2021 | FC Twente | - | Heracles Almelo | 1:1 | ||||
| 33. Round | 13/05/2021 | RKC Waalwijk | - | FC Twente | 2:1 | ||||
| 34. Round | 16/05/2021 | FC Twente | - | ADO Den Haag | 3:2 |
Netherlands