| 1. Round | 09/08/2024 | Sokol Hostouň | - | Dynamo České Budějovice B | 0:0 | ||||
| 2. Round | 17/08/2024 | Dynamo České Budějovice B | - | Slovan Velvary | 0:6 | ||||
| 3. Round | 25/08/2024 | Viktoria Plzeň B | - | Dynamo České Budějovice B | 0:3 | ||||
| 17. Round | 28/08/2024 | FK Příbram | - | Dynamo České Budějovice B | 2:1 | ||||
| 4. Round | 31/08/2024 | Dynamo České Budějovice B | - | FK Motorlet Praha | 3:2 | ||||
| 5. Round | 08/09/2024 | FK Admira Praha | - | Dynamo České Budějovice B | 3:6 |  |
|||
| 7. Round | 21/09/2024 | FK Loko Vltavín | - | Dynamo České Budějovice B | 2:4 | ||||
| 8. Round | 28/09/2024 | Dynamo České Budějovice B | - | SK Petřín Plzeň | 2:2 |  |
|||
| 9. Round | 05/10/2024 | TJ Jiskra Domažlice | - | Dynamo České Budějovice B | 4:4 | |
|||
| 10. Round | 12/10/2024 | Dynamo České Budějovice B | - | FK MAS Táborsko B | 1:2 | ||||
| 6. Round | 17/10/2024 | Dynamo České Budějovice B | - | Bohemians Praha 1905 B | 3:2 | ||||
| 12. Round | 26/10/2024 | Dynamo České Budějovice B | - | FC Písek | 2:0 | ||||
| 13. Round | 01/11/2024 | FC Chomutov | - | Dynamo České Budějovice B | 1:0 | |
|||
| 14. Round | 09/11/2024 | Dynamo České Budějovice B | - | FK Dukla Praha B | 2:1 | |
|
Czech Republic