| 1. Round | 27/07/2024 | KFC Uerdingen 05 | - | Sportfreunde Lotte | 0:2 | ||||
| 2. Round | 02/08/2024 | Sportfreunde Lotte | - | FC Gütersloh | 3:2 | ||||
| 3. Round | 10/08/2024 | Türkspor Dortmund | - | Sportfreunde Lotte | 0:1 | ||||
| 5. Round | 24/08/2024 | Eintracht Hohkeppel | - | Sportfreunde Lotte | 2:2 |  |
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| 6. Round | 01/09/2024 | Sportfreunde Lotte | - | SC Paderborn 07 II | 3:2 | ||||
| 4. Round | 07/09/2024 | Sportfreunde Lotte | - | Rot-Weiß Oberhausen | 1:4 | |
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| 7. Round | 15/09/2024 | Fortuna Düsseldorf II | - | Sportfreunde Lotte | 1:4 | ||||
| 8. Round | 21/09/2024 | Sportfreunde Lotte | - | Fortuna Köln | 1:1 | ||||
| 9. Round | 27/09/2024 | SC Wiedenbrück | - | Sportfreunde Lotte | 2:4 | ||||
| 10. Round | 05/10/2024 | Sportfreunde Lotte | - | FC Schalke 04 II | 4:1 | ||||
| 11. Round | 12/10/2024 | SV Rödinghausen | - | Sportfreunde Lotte | 2:3 | ||||
| 12. Round | 19/10/2024 | Sportfreunde Lotte | - | 1. FC Bocholt | 4:1 | ||||
| 13. Round | 26/10/2024 | 1. FC Düren | - | Sportfreunde Lotte | 2:1 | ||||
| 15. Round | 09/11/2024 | Bor. Mönchengladbach II | - | Sportfreunde Lotte | 1:2 |
Germany