| 1. Round | 03/08/2024 | Hanácká Slávia Kroměříž | - | TJ Start Brno | 2:0 |  |
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| 2. Round | 11/08/2024 | TJ Start Brno | - | TJ Unie Hlubina | 1:1 | ||||
| 3. Round | 16/08/2024 | FK Třinec | - | TJ Start Brno | 2:1 |  |
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| 4. Round | 23/08/2024 | TJ Start Brno | - | FK Hodonín | 2:2 | ||||
| 5. Round | 01/09/2024 | 1. FC Slovácko B | - | TJ Start Brno | 3:2 | |
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| 6. Round | 07/09/2024 | TJ Start Brno | - | FK Frýdek-Místek | 2:1 | |
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| 17. Round | 11/09/2024 | TJ Start Brno | - | FC Strání | 2:0 | |
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| 8. Round | 22/09/2024 | 1. SC Znojmo | - | TJ Start Brno | 2:3 | |
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| 9. Round | 27/09/2024 | TJ Start Brno | - | FC Hlučín | 0:0 | ||||
| 7. Round | 01/10/2024 | FC Slovan Rosice | - | TJ Start Brno | 0:0 | ||||
| 10. Round | 06/10/2024 | MFK Karviná B | - | TJ Start Brno | 5:2 | |
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| 11. Round | 12/10/2024 | TJ Start Brno | - | FC Zlín B | 0:0 | |
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| 12. Round | 20/10/2024 | FC Zbrojovka Brno B | - | TJ Start Brno | 1:2 | ||||
| 13. Round | 27/10/2024 | TJ Start Brno | - | FK Blansko | 2:2 | |
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| 14. Round | 03/11/2024 | ČSK Uherský Brod | - | TJ Start Brno | 2:2 | |
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| 15. Round | 10/11/2024 | TJ Start Brno | - | FC Zlínsko | 1:1 | ||||
| 16. Round | 16/11/2024 | SK Uničov | - | TJ Start Brno | 3:0 |
Czech Republic