| 27. Round | 16/01/2010 | Hartlepool United | - | Milton Keynes Dons | 0:5 | ||||
| 25. Round | 26/01/2010 | Colchester United | - | Milton Keynes Dons | 2:0 |  |
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| 29. Round | 30/01/2010 | Milton Keynes Dons | - | Exeter City | 1:1 | ||||
| 35. Round | 06/03/2010 | Milton Keynes Dons | - | Leyton Orient | 1:0 | |
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| 26. Round | 09/03/2010 | Milton Keynes Dons | - | Swindon Town | 2:1 | ||||
| 36. Round | 13/03/2010 | Brentford FC | - | Milton Keynes Dons | 3:3 | |
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| 37. Round | 20/03/2010 | Milton Keynes Dons | - | Southampton FC | 0:3 | ||||
| 38. Round | 27/03/2010 | Gillingham FC | - | Milton Keynes Dons | 2:2 | ||||
| 39. Round | 03/04/2010 | Milton Keynes Dons | - | Charlton Athletic | 0:1 | ||||
| 40. Round | 05/04/2010 | Bristol Rovers | - | Milton Keynes Dons | 1:0 | |
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| 41. Round | 10/04/2010 | Norwich City | - | Milton Keynes Dons | 1:1 | |
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| 42. Round | 13/04/2010 | Milton Keynes Dons | - | Oldham Athletic | 0:0 | ||||
| 43. Round | 17/04/2010 | Milton Keynes Dons | - | Wycombe Wanderers | 2:3 | ||||
| 44. Round | 24/04/2010 | Leeds United | - | Milton Keynes Dons | 4:1 | |
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| 45. Round | 01/05/2010 | Milton Keynes Dons | - | Brighton & Hove Albion | 0:0 | ||||
| 46. Round | 08/05/2010 | Walsall FC | - | Milton Keynes Dons | 2:1 |
England