| 1. Round | 26/08/2023 | FC Hradec Králové [Women] | - | AC Sparta Praha B [Women] | 2:5 |  |
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| 2. Round | 03/09/2023 | AC Sparta Praha B [Women] | - | Sigma Olomouc [Women] | 7:0 | ||||
| 3. Round | 09/09/2023 | FK Dukla Praha [Women] | - | AC Sparta Praha B [Women] | 1:2 | ||||
| 4. Round | 16/09/2023 | AC Sparta Praha B [Women] | - | FK Teplice [Women] | 8:0 | |
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| 5. Round | 23/09/2023 | FC Praha [Women] | - | AC Sparta Praha B [Women] | 0:9 | ||||
| 6. Round | 30/09/2023 | AC Sparta Praha B [Women] | - | SK Líšeň [Women] | 3:2 | ||||
| 7. Round | 07/10/2023 | Prague Raptors FC [Women] | - | AC Sparta Praha B [Women] | 2:2 | |
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| 8. Round | 15/10/2023 | AC Sparta Praha B [Women] | - | FC Hradec Králové [Women] | 7:0 | |
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| 9. Round | 21/10/2023 | Sigma Olomouc [Women] | - | AC Sparta Praha B [Women] | 0:3 | |
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| 10. Round | 28/10/2023 | AC Sparta Praha B [Women] | - | FK Dukla Praha [Women] | 4:1 | |
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| 11. Round | 23/03/2024 | FK Teplice [Women] | - | AC Sparta Praha B [Women] | 0:1 |  |
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| 12. Round | 30/03/2024 | AC Sparta Praha B [Women] | - | FC Praha [Women] | 2:3 |
Czech Republic