| 17. Round | 19/02/2000 | Slovan Liberec | - | FC Dukla Pribram | 0:0 | ||||
| 18. Round | 27/02/2000 | FC Dukla Pribram | - | FK Teplice | 3:0 | ||||
| 19. Round | 05/03/2000 | Chmel Blsany | - | FC Dukla Pribram | 2:1 | ||||
| 20. Round | 11/03/2000 | FC Dukla Pribram | - | AC Sparta Praha | 1:3 |  |
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| 21. Round | 20/03/2000 | Slavia Praha | - | FC Dukla Pribram | 3:1 | |
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| 22. Round | 25/03/2000 | FC Dukla Pribram | - | Dynamo České Budějovice | 1:0 | ||||
| 23. Round | 02/04/2000 | Sigma Olomouc | - | FC Dukla Pribram | 1:0 | ||||
| 24. Round | 09/04/2000 | FC Dukla Pribram | - | FC Boby-sport Brno | 1:2 | ||||
| 25. Round | 16/04/2000 | FC Petra Drnovice | - | FC Dukla Pribram | 2:1 | ||||
| 26. Round | 22/04/2000 | FC Dukla Pribram | - | CU Bohemians Praha | 3:0 | ||||
| 27. Round | 30/04/2000 | Viktoria Žižkov | - | FC Dukla Pribram | 1:1 |  |
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| 28. Round | 07/05/2000 | FC Dukla Pribram | - | Baník Ostrava | 2:0 | ||||
| 29. Round | 13/05/2000 | SFC Opava | - | FC Dukla Pribram | 0:1 | ||||
| 30. Round | 17/05/2000 | FC Dukla Pribram | - | SK Hradec Králové | 1:0 |
Czech Republic