| 1. Round | 10/08/2024 | Gateshead FC | - | Ebbsfleet United | 5:1 |  |
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| 2. Round | 17/08/2024 | Ebbsfleet United | - | Barnet FC | 1:2 | |
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| 3. Round | 20/08/2024 | Yeovil Town | - | Ebbsfleet United | 3:2 | ||||
| 4. Round | 24/08/2024 | Ebbsfleet United | - | Woking FC | 0:3 | ||||
| 5. Round | 26/08/2024 | Braintree Town | - | Ebbsfleet United | 0:0 | ||||
| 6. Round | 31/08/2024 | Ebbsfleet United | - | Halifax Town | 0:1 | ||||
| 7. Round | 07/09/2024 | Altrincham FC | - | Ebbsfleet United | 4:0 | ||||
| 8. Round | 10/09/2024 | Ebbsfleet United | - | Aldershot Town | 0:0 | ||||
| 10. Round | 21/09/2024 | Sutton United | - | Ebbsfleet United | 3:2 | |
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| 11. Round | 24/09/2024 | Maidenhead United | - | Ebbsfleet United | 2:1 | ||||
| 12. Round | 28/09/2024 | Ebbsfleet United | - | Oldham Athletic | 1:2 | |
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| 13. Round | 05/10/2024 | Ebbsfleet United | - | Rochdale AFC | 2:2 | ||||
| 14. Round | 19/10/2024 | York City | - | Ebbsfleet United | 4:0 | ||||
| 15. Round | 22/10/2024 | Forest Green Rovers | - | Ebbsfleet United | 3:1 | ||||
| 16. Round | 26/10/2024 | Ebbsfleet United | - | Wealdstone FC | 2:2 |
England