| 1. Round | 20/07/2024 | MFK Vyškov | - | 1. SK Prostějov | 0:0 | ||||
| 2. Round | 26/07/2024 | Vysočina Jihlava | - | MFK Vyškov | 1:1 | ||||
| 3. Round | 31/07/2024 | MFK Vyškov | - | FC Sellier & Bellot Vlašim | 1:1 | ||||
| 4. Round | 04/08/2024 | Baník Ostrava B | - | MFK Vyškov | 1:3 | ||||
| 5. Round | 12/08/2024 | MFK Vyškov | - | Slavia Praha B | 1:0 | ||||
| 6. Round | 16/08/2024 | SFC Opava | - | MFK Vyškov | 1:0 |  |
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| 7. Round | 26/08/2024 | MFK Vyškov | - | Zbrojovka Brno | 1:0 | ||||
| 8. Round | 01/09/2024 | FK Varnsdorf | - | MFK Vyškov | 1:2 | ||||
| 10. Round | 21/09/2024 | AC Sparta Praha B | - | MFK Vyškov | 1:0 | ||||
| 11. Round | 28/09/2024 | FK MAS Táborsko | - | MFK Vyškov | 0:0 | ||||
| 9. Round | 02/10/2024 | MFK Vyškov | - | MFK Chrudim | 0:3 | ||||
| 12. Round | 06/10/2024 | MFK Vyškov | - | Sigma Olomouc B | 1:0 | ||||
| 13. Round | 19/10/2024 | SK Líšeň | - | MFK Vyškov | 0:2 | ||||
| 14. Round | 26/10/2024 | MFK Vyškov | - | FC Zlín | 1:1 | ||||
| 15. Round | 03/11/2024 | Viktoria Žižkov | - | MFK Vyškov | 0:3 | ||||
| 16. Round | 10/11/2024 | MFK Vyškov | - | Vysočina Jihlava | 1:1 |
Czech Republic